10 WALZ Imaging PAM (Maxi and Microscopy)
Image gallery:
Contact:
University Innsbruck, Sternwartestrasse 15, Innsbruck, A-6020
Email: thomas.roach@uibk.ac.at
Web: https://www.uibk.ac.at/en/department-of-botany/research/stress-metabolism/
Key facts:
Description:
| Name | WALZ Imaging PAM (Maxi and Microscopy) |
|---|---|
| Location | University Innsbruck, Department of Botany, Sternwartestrasse 15, Innsbruck, A-6020 |
| Category | Bench top |
| Environment | Lab / growth chamber |
| Sensors | PAM Chlorophyll fluorescence |
| Traits | Fv/Fm, NPQ, ETR(II) |
| Capacity | Medium throughput (96-well plate possible) |
| Limitations | 1,200µE actinic light |
| References | |
| URL | https://www.walz.com/products/chl_p700/imaging-pam/introduction.html |
The MAXI version of the IMAGING-PAM M-Series employs a very compact and powerful 300 W LED array for the homogeneous illumination of 10 x 13 cm areas (microtiter plates) with pulse-modulated excitation, actinic light and saturation pulses. On the bottom of the Mounting Stand an x-y stage for variable sample positioning or a multiwell plate can be placed at defined working distance of 18.5 cm.
The MICROSCOPY version of the IMAGING-PAM M-Series features imaging analysis on single cell level. Providing high sensitivity in conjunction with a modified Axioscope 5 (Zeiss, Göttingen) epifluorescence microscopes and the IMAG-K6 CCD Camera (2/3″ chip with 1392 x 1040 pixels primary resolution and 4-pixel-binning). The ImagingWin software takes account of the particular needs of microscopy applications. A life video image of the object can be obtained using the standard through-light condenser-illuminator of the microscope. A special saturation pulse routine is provided for optimal assessment of Fo, Fm and Fv/Fm at low levels of excitation intensity. For standard applications a single high power Luxeon LED (470 nm) is provided for excitation, actinic illumination and saturation pulses.